• Open access free of charge
    • Free and high quality figure editing
    • Free widest possible global promotion for your research
Volume 5 Issue 4
Jul.  2023
Article Contents

Zhou D Z, Dou B H, Kroh F, Wang C Q, Ouyang L L. 2023. Biofabrication strategies with single-cell resolution: a review. Int. J. Extrem. Manuf. 5 042005.
Citation: Zhou D Z, Dou B H, Kroh F, Wang C Q, Ouyang L L. 2023. Biofabrication strategies with single-cell resolution: a review. Int. J. Extrem. Manuf. 042005.

Biofabrication strategies with single-cell resolution: a review


doi: 10.1088/2631-7990/ace863
More Information
  • Publish Date: 2023-07-26
  • The introduction of living cells to manufacturing process has enabled the engineering of complex biological tissues in vitro. The recent advances in biofabrication with extremely high resolution (e.g. at single cell level) have greatly enhanced this capacity and opened new avenues for tissue engineering. In this review, we comprehensively overview the current biofabrication strategies with single-cell resolution and categorize them based on the dimension of the single-cell building blocks, i.e. zero-dimensional single-cell droplets, one-dimensional single-cell filaments and two-dimensional single-cell sheets. We provide an informative introduction to the most recent advances in these approaches (e.g. cell trapping, bioprinting, electrospinning, microfluidics and cell sheets) and further illustrated how they can be used in in vitro tissue modelling and regenerative medicine. We highlight the significance of single-cell-level biofabrication and discuss the challenges and opportunities in the field.

  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Figures(1)

Article Metrics

Article views(172) PDF Downloads(7) Citation(0)

Biofabrication strategies with single-cell resolution: a review

doi: 10.1088/2631-7990/ace863
  • 1 Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China;
  • 2 Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Tsinghua University, Beijing 100084, People's Republic of China;
  • 3 ‘Biomanufacturing and Engineering Living Systems’ Innovation International Talents Base(111 Base), Tsinghua University, Beijing 100084, People's Republic of China

Abstract: 

The introduction of living cells to manufacturing process has enabled the engineering of complex biological tissues in vitro. The recent advances in biofabrication with extremely high resolution (e.g. at single cell level) have greatly enhanced this capacity and opened new avenues for tissue engineering. In this review, we comprehensively overview the current biofabrication strategies with single-cell resolution and categorize them based on the dimension of the single-cell building blocks, i.e. zero-dimensional single-cell droplets, one-dimensional single-cell filaments and two-dimensional single-cell sheets. We provide an informative introduction to the most recent advances in these approaches (e.g. cell trapping, bioprinting, electrospinning, microfluidics and cell sheets) and further illustrated how they can be used in in vitro tissue modelling and regenerative medicine. We highlight the significance of single-cell-level biofabrication and discuss the challenges and opportunities in the field.

Reference (265)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return